Download E-books Assay Development: Fundamentals and Practices PDF

By Ge Wu

Essential rules and perform of assay development

The first finished, built-in remedy of the topic, Assay improvement: basics and Practices covers the necessities and methods fascinated about conducting an assay venture in both a biotechnology/drug discovery surroundings or a platform setting.

Rather than trying accomplished assurance of all assay improvement applied sciences, the ebook introduces the main generic assay improvement applied sciences and illustrates the artwork of assay improvement via a couple of as a rule encountered organic objectives in assay improvement (e.g., proteases, kinases, ion channels, and G protein-coupled receptors). simply enough organic history for those organic objectives is supplied in order that the reader can keep on with the logics of assay improvement. Chapters discuss:

  • The fundamentals of assay improvement, together with foundational strategies and applications

  • Commonly used instrumental tools for either biochemical assays and cell-based assays

  • Assay techniques for protein binding and enzymatic activity

  • Cell-based assays

  • High-throughput screening

An in-depth learn of the now renowned Caliper's off-chip kinase assay presents an instructive, real-world instance of the assay improvement process.

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