By Charles A. Lucy, Nicole E. Baryla (auth.), Mark A. Strege, Avinash L. Lagu (eds.)
Capillary electrophoresis (CE) is without doubt one of the most advantageous tools for the speedy research of pharmaceutical proteins and peptides, and hence now much more very important with the sequencing of the human genome and the next introduction of proteomics. In Capillary Electrophoresis of Proteins and Peptides, top educational scientists and professional laboratorians operating within the pharmaceutical describe their top CE suggestions in step by step aspect for simple replica. The authors current state-of-the-art concepts for either capillary coatings and analytical detection through laser-induced fluorescence, for the advance and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis within the overview of protein binding, together with using protein cost ladders. extra chapters speak about CE and capillary isoelectric focusing, mixed with electrospray mass spectrometry detection, for appearing proteomic reports. parts of particular curiosity lined contain proteomic functions, in addition to functions within the box of protein-ligand binding. The protocols stick to the winning tools in Molecular Biology™ sequence layout, each one supplying step by step laboratory directions, an advent outlining the primary in the back of the process, lists of kit and reagents, and pointers on troubleshooting and warding off identified pitfalls.
finished and cutting-edge, Capillary Electrophoresis of Proteins and Peptides will end up necessary to either new and skilled investigators conducting major protein and peptide reports in biotechnology, drug discovery, and pharmaceutical examine and development.
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