This profitable laboratory guide makes a speciality of tactics for hybridoma new release and provides unique try protocols for the applying of monoclonal antibodies. Immunization, mobile education, hybridization, telephone tradition and cloning of hybridomas, prevention of back-mutation, in addition to purification, labeling, and characterization of the antibodies received, are just a couple of key phrases concerning the contents. useful tricks and targeted product info including distinctive protocols for trouble-shooting make this booklet a very necessary "cookbook" in addition to a compendium of immunological equipment over and above monoclonal antibody expertise. The authors have emphasised protocols which are inclined to reduce the variety of animals used and want tools which stay away from radioactivity.
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